Rat peritoneal mast cells release dipeptidyl peptidase II
نویسندگان
چکیده
منابع مشابه
Rat peritoneal mast cells release dipeptidyl peptidase II.
Purified rat peritoneal mast cells have a 10-20-fold higher dipeptidyl peptidase II (DPP II) activity as compared with that of macrophages from the same source. Upon stimulation with the secretagogue Compound 48/80, DPP II is released from peritoneal-lavage cells and from purified mast cells, but not from purified macrophages, in a dose-dependent manner. Maximally, about one-third of the DPP II...
متن کاملNative heparin from rat peritoneal mast cells.
[35S]Heparin was produced in vitro by incubation of rat peritoneal mast cells with [35S]sulfate and in vivo by injection of [35S]sulfate into rats. The [35S]heparin together with nonlabeled heparin in the mast cells was isolated in native form by mild methods that avoided the use of proteolytic enzymes or high alkali concentrations. The heparin had low anticoagulant activity. Incubations of mas...
متن کاملRespiration of Rat Peritoneal Mast Cells
Methods for microgasometry of a few hundred mast cells are described. The Cartesian ampulla diver technique is used. The sample size is determined by counting the cells within the diver. The respiration rates at 37 degrees C, expressed in microliters per cell per hour, are 0.29 x 10(-6) without substrate and 0.47 x 10(-6) with glucose.
متن کاملGlycolysis in Rat Peritoneal Mast Cells
Glycolytic activity of rat peritoneal mast cells has been measured by the Cartesian ampulla diver technique. The rates of anaerobic glycolysis, expressed as CO(2) expelled from a bicarbonate medium, are 1.70 x 10(-6) microl and 1.43 x 10(-6) microl per cell per hour with and without glucose, respectively. The aerobic glycolysis rate in the presence of glucose, assuming the respiratory quotient ...
متن کامل[Naturally occurring aliphatic polyamines-induced histamine release from rat peritoneal mast cells].
Rat peritoneal mast cells obtained by Percoll gradient were incubated with different concentrations of naturally occurring aliphatic polyamines, spermine and spermidine, at 0.1-10 mM and the amount of histamine release into the supernatant solutions was measured. The addition of each polyamine to the suspensions of the mast cells caused a histamine release in a dose-dependent manner. The histam...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2360215